|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Mouse CPB2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50963-ACG|
|Mouse CPB2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50963-ACR|
|Mouse CPB2 ORF mammalian expression plasmid, C-Flag tag||MG50963-CF|
|Mouse CPB2 ORF mammalian expression plasmid, C-His tag||MG50963-CH|
|Mouse CPB2 ORF mammalian expression plasmid, C-Myc tag||MG50963-CM|
|Mouse CPB2 ORF mammalian expression plasmid, C-HA tag||MG50963-CY|
|Mouse CPB2 Gene cDNA clone plasmid||MG50963-G|
|Mouse CPB2 ORF mammalian expression plasmid, N-Flag tag||MG50963-NF|
|Mouse CPB2 ORF mammalian expression plasmid, N-His tag||MG50963-NH|
|Mouse CPB2 ORF mammalian expression plasmid, N-Myc tag||MG50963-NM|
|Mouse CPB2 ORF mammalian expression plasmid, N-HA tag||MG50963-NY|
|Mouse CPB2 natural ORF mammalian expression plasmid||MG50963-UT|
|Learn more about expression Vectors|
Carboxypeptidase B2, also known as Carboxypeptidase U, Thrombin-activable fibrinolysis inhibitor, Plasma carboxypeptidase B, CPB2, is a secreted protein which belongs to the peptidase M14 family. Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). CPB2 is activated by thrombin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin / thrombomodulin complex. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. CPB2 exhibits carboxypeptidase activity and activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.