|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat PCSK9 ORF mammalian expression plasmid, C-GFPSpark tag||RG80005-ACG|
|Rat PCSK9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80005-ACR|
|Rat PCSK9 ORF mammalian expression plasmid, C-Flag tag||RG80005-CF|
|Rat PCSK9 ORF mammalian expression plasmid, C-His tag||RG80005-CH|
|Rat PCSK9 ORF mammalian expression plasmid, C-Myc tag||RG80005-CM|
|Rat PCSK9 ORF mammalian expression plasmid, C-HA tag||RG80005-CY|
|Rat PCSK9 Gene cDNA clone plasmid||RG80005-M|
|Rat PCSK9 ORF mammalian expression plasmid, N-Flag tag||RG80005-NF|
|Rat PCSK9 ORF mammalian expression plasmid, N-His tag||RG80005-NH|
|Rat PCSK9 ORF mammalian expression plasmid, N-Myc tag||RG80005-NM|
|Rat PCSK9 ORF mammalian expression plasmid, N-HA tag||RG80005-NY|
|Rat PCSK9 natural ORF mammalian expression plasmid||RG80005-UT|
|Learn more about expression Vectors|
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.