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Rat PON3 Gene cDNA clone plasmid

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Rat PON3 cDNA Clone Product Information
Gene_bank_ref_id:NM_001004086.1
RefSeq ORF Size:1065bp
cDNA Description:Full length Clone DNA of Rattus norvegicus paraoxonase 3.
Gene Synonym:Pon3
Species:Rat
Vector:pUC19 Vector
Plasmid:cpUC19-ratPon3
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 85A>G(S29G) and 316T>G(F106V).
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Rat PON3 Gene Plasmid Map
Rat PON3 Gene cDNA Clone (full-length ORF Clone)
pUC19 vector Vector Information:

pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.

pUC19 vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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References
  • Draganov DI, et al. (2000) Rabbit serum paraoxonase 3 (PON3) is a high density lipoprotein-associated lactonase and protects low density lipoprotein against oxidation. J Biol Chem. 275(43): 33435-42.
  • Shih DM, et al. (2007) Decreased obesity and atherosclerosis in human paraoxonase 3 transgenic mice. Circ Res. 100(8): 1200-7.
  • Rosenblat M, et al. (2003) Mouse macrophage paraoxonase 2 activity is increased whereas cellular paraoxonase 3 activity is decreased under oxidative stress. Arterioscler Thromb Vasc Biol. 23(3): 468-74.
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    Catalog: RG81169-U
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    • Rat PON3 Gene cDNA Clone (full-length ORF Clone)
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"