|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Mouse STIM1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50955-ACG|
|Mouse STIM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50955-ACR|
|Mouse STIM1 ORF mammalian expression plasmid, C-Flag tag||MG50955-CF|
|Mouse STIM1 ORF mammalian expression plasmid, C-His tag||MG50955-CH|
|Mouse STIM1 ORF mammalian expression plasmid, C-Myc tag||MG50955-CM|
|Mouse STIM1 ORF mammalian expression plasmid, C-HA tag||MG50955-CY|
|Mouse STIM1 Gene cDNA clone plasmid||MG50955-G|
|Mouse STIM1 ORF mammalian expression plasmid, N-Flag tag||MG50955-NF|
|Mouse STIM1 ORF mammalian expression plasmid, N-His tag||MG50955-NH|
|Mouse STIM1 ORF mammalian expression plasmid, N-Myc tag||MG50955-NM|
|Mouse STIM1 ORF mammalian expression plasmid, N-HA tag||MG50955-NY|
|Mouse STIM1 natural ORF mammalian expression plasmid||MG50955-UT|
|Learn more about expression Vectors|
Stromal interaction molecule 1, also known as STIM1 and GOK, is a cell membrane, a single-pass type I membrane protein and a endoplasmic reticulum membrane protein. STIM1 / GOK is ubiquitously expressed in various human primary cells and tumor cell lines. It contains one EF-hand domain and one SAM (sterile alpha motif) domain. STIM1 / GOK plays a role in mediating Ca2+ influx following depletion of intracellular Ca2+ stores. It acts as Ca2+ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca2+ depletion, STIM1 / GOK translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit, TMEM142A / ORAI1. Transfection of STIM1 / GOK into cells derived from a rhabdoid tumor and from a rhabdomyosarcoma that do not express detectable levels of STIM1 can induce cell death, suggesting a possible role in the control of rhabdomyosarcomas and rhabdoid tumors. Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) which is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels.