After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!

Quick Order

Text Size:AAA

Influenza A H1N1 (Beijing/22808/2009) Hemagglutinin / HA ORF mammalian expression plasmid (Codon Optimized), C-Flag tag

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
H1N1 HA cDNA Clone Product Information
Gene_bank_ref_id:
RefSeq ORF Size:1701bp
cDNA Description:Full length Clone DNA of Influenza A H1N1 (A/Beijing/22808/2009) Hemagglutinin with C terminal Flag tag.
Gene Synonym:Hemagglutinin, HA
Species:H1N1
Vector:pCMV3-C-FLAG
Plasmid:
Restriction Site:
Tag Sequence:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequence Description:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with ADD64203.1.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Influenza A H1N1 (Beijing/22808/2009) Hemagglutinin / HA ORF mammalian expression plasmid (Codon Optimized), C-Flag tag on other vectors
Influenza A H1N1 (A/Beijing/22808/2009) Hemagglutinin / HA ORF mammalian expression plasmid (Codon Optimized), C-GFPSpark-taggedVG40035-ACG$345
Influenza A H1N1 (A/Beijing/22808/2009) Hemagglutinin / HA (Codon Optimized) ORF mammalian expression plasmid, C-OFPSpark / RFP tagVG40035-ACR$345
Influenza A H1N1 (A/Beijing/22808/2009) Hemagglutinin / HA ORF mammalian expression plasmid (Codon Optimized)VG40035-C$395
Influenza A H1N1 (A/Beijing/22808/2009) Hemagglutinin / HA (Codon Optimized) ORF mammalian expression plasmid, Myc tagVG40035-C-M$395
Influenza A H1N1 (Beijing/22808/2009) Hemagglutinin / HA ORF mammalian expression plasmid (Codon Optimized), C-Flag tagVG40035-CF$315
Influenza A H1N1 (Beijing/22808/2009) Hemagglutinin / HA (Codon Optimized) ORF mammalian expression plasmid, C-His tagVG40035-CH$315
Influenza A H1N1 (Beijing/22808/2009) Hemagglutinin / HA (Codon Optimized) ORF mammalian expression plasmid, C-Myc tagVG40035-CM$315
Influenza A H1N1 (Beijing/22808/2009) Hemagglutinin / HA (Codon Optimized) ORF mammalian expression plasmid, C-HA tagVG40035-CY$315
Influenza A H1N1 (Beijing/22808/2009) Hemagglutinin / HA (Codon Optimized) ORF mammalian expression plasmid, N-Flag tagVG40035-NF$315
Influenza A H1N1 (Beijing/22808/2009) Hemagglutinin / HA (Codon Optimized) ORF mammalian expression plasmid, N-His tagVG40035-NH$315
Influenza A H1N1 (Beijing/22808/2009) Hemagglutinin / HA ORF mammalian expression plasmid (Codon Optimized), N-Myc-taggedVG40035-NM$315
Influenza A H1N1 (Beijing/22808/2009) Hemagglutinin / HA (Codon Optimized) ORF mammalian expression plasmid, N-HA tagVG40035-NY$315
Influenza A H1N1 (Beijing/22808/2009) Hemagglutinin / HA ORF mammalian expression plasmid (Codon Optimized)VG40035-UT$315
 Learn more about expression Vectors
Product nameProduct name
Size / Price
Catalog: VG40035-CF
List Price:   (Save )
Price:      [How to order]
Availability2-3 weeksShipping instructions
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"