|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse LGALS1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50100-ACG|
|Mouse LGALS1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50100-ACR|
|Mouse LGALS1 ORF mammalian expression plasmid, C-Flag tag||MG50100-CF|
|Mouse LGALS1 ORF mammalian expression plasmid, C-His tag||MG50100-CH|
|Mouse LGALS1 ORF mammalian expression plasmid, C-Myc tag||MG50100-CM|
|Mouse LGALS1 ORF mammalian expression plasmid, C-HA tag||MG50100-CY|
|Mouse LGALS1 Gene cDNA clone plasmid||MG50100-M|
|Mouse LGALS1 ORF mammalian expression plasmid, N-Flag tag||MG50100-NF|
|Mouse LGALS1 ORF mammalian expression plasmid, N-His tag||MG50100-NH|
|Mouse LGALS1 ORF mammalian expression plasmid, N-Myc tag||MG50100-NM|
|Mouse LGALS1 ORF mammalian expression plasmid, N-HA tag||MG50100-NY|
|Mouse LGALS1 natural ORF mammalian expression plasmid||MG50100-UT|
|Learn more about expression Vectors|
Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state in order to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumour growth, has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumour progression including cell adhesion, migration and tumour-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.