|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse JTB ORF mammalian expression plasmid, C-GFPSpark tag||MG52774-ACG|
|Mouse JTB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52774-ACR|
|Mouse JTB ORF mammalian expression plasmid, C-Flag tag||MG52774-CF|
|Mouse JTB ORF mammalian expression plasmid, C-His tag||MG52774-CH|
|Mouse JTB ORF mammalian expression plasmid, C-Myc tag||MG52774-CM|
|Mouse JTB ORF mammalian expression plasmid, C-HA tag||MG52774-CY|
|Mouse JTB Gene cDNA clone plasmid||MG52774-G|
|Mouse JTB ORF mammalian expression plasmid, N-Flag tag||MG52774-NF|
|Mouse JTB ORF mammalian expression plasmid, N-His tag||MG52774-NH|
|Mouse JTB ORF mammalian expression plasmid, N-Myc tag||MG52774-NM|
|Mouse JTB ORF mammalian expression plasmid, N-HA tag||MG52774-NY|
|Mouse JTB natural ORF mammalian expression plasmid||MG52774-UT|
|Learn more about expression Vectors|
Jumping translocation breakpoint, also known as JTB, is a member of the JTB family. Jumping translocation (JT) is an unbalanced translocation that comprises amplified chromosomalsegments jumping to various telomeres. JTB is expressed in all normal human tissues studied but overexpressed or underexpressed in many of their malignant counterparts. It is required for normal cytokinesis during mitosis. JTB plays a role in the regulation of cell proliferation. It may be a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly.