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Human TGFA / TGF-alpha Protein

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Human TGFA Protein Product Information
Synonym:TGFa
Protein Construction:A DNA sequence encoding the human TGFa (P01135-1) (Val40-Ala89) was expressed and purified.
Species:Human
Expressed Host:E. coli
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human TGFA Protein QC Testing
Purity:> 90 % as determined by SDS-PAGE
Bio-Activity:1. Measured by its binding ability in a functional ELISA. Immobilized human TGFa (cat:11252-HNAE) at 10 μg/ml (100 μl/well) can bind human EGFR-Fc (Cat:10001-H02H). The EC50 of human EGFR-Fc (Cat:10001-H02H) is 20-60 ng/ml.
2. Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.
The ED50 for this effect is 0.1-0.5ng/mL.
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human TGFa consists of 50 amino acids and has a predicted molecular mass of 5.6 kDa. The apparent molecular mass of it is approximately 13 kDa in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human TGFA Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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11252-HNAE
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Size / Price
Catalog: 11252-HNAE-500
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"