|Datasheet||Specific References||Reviews||Related Products||Protocols|
|A DNA sequence encoding the human TGFa (P01135-1) (Val40-Ala89) was expressed and purified.|
|In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|> 90 % as determined by SDS-PAGE|
|1. Measured by its binding ability in a functional ELISA. Immobilized human TGFa (cat:11252-HNAE) at 10 μg/ml (100 μl/well) can bind human EGFR-Fc (Cat:10001-H02H). The EC50 of human EGFR-Fc (Cat:10001-H02H) is 20-60 ng/ml.|
2. Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.
The ED50 for this effect is 0.1-0.5ng/mL.
|Please contact us for more information.|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|The recombinant human TGFa consists of 50 amino acids and has a predicted molecular mass of 5.6 kDa. The apparent molecular mass of it is approximately 13 kDa in SDS-PAGE under reducing conditions.|
|Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0.|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|