|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse ASAH2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50006-ACG|
|Mouse ASAH2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50006-ACR|
|Mouse ASAH2 ORF mammalian expression plasmid, C-Flag tag||MG50006-CF|
|Mouse ASAH2 ORF mammalian expression plasmid, C-His tag||MG50006-CH|
|Mouse ASAH2 ORF mammalian expression plasmid, C-Myc tag||MG50006-CM|
|Mouse ASAH2 ORF mammalian expression plasmid, C-HA tag||MG50006-CY|
|Mouse ASAH2 Gene cDNA clone plasmid||MG50006-M|
|Mouse ASAH2 ORF mammalian expression plasmid, N-Flag tag||MG50006-NF|
|Mouse ASAH2 ORF mammalian expression plasmid, N-His tag||MG50006-NH|
|Mouse ASAH2 ORF mammalian expression plasmid, N-Myc tag||MG50006-NM|
|Mouse ASAH2 ORF mammalian expression plasmid, N-HA tag||MG50006-NY|
|Mouse ASAH2 natural ORF mammalian expression plasmid||MG50006-UT|
|Learn more about expression Vectors|
ASAH2 (N-acylsphingosine amidohydrolase 2), also known as neutral ceramidase, is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in the brush border membranes of the intestine, and also expressed in several tissues such as kidney, brain and liver. The primary structure of ASAH2/neutral ceramidase is highly conserved from bacteria to humans, however, there is a clear difference in the molecular architecture. The murine ASAH2 possesses ‘amucin box’, a Ser/Thr/Pro-rich domain glycosylated with O-glycans which is necessary to retain the enzyme on the plasma membrane as a type II integral protein. The major physiological function of ASAH2/neutral ceramidase is the metabolism of dietary sphingolipids, and thus plays a role in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.