|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CLDN11 ORF mammalian expression plasmid, C-GFPSpark tag||RG81317-ACG|
|Rat CLDN11 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81317-ACR|
|Rat CLDN11 ORF mammalian expression plasmid, C-Flag tag||RG81317-CF|
|Rat CLDN11 ORF mammalian expression plasmid, C-His tag||RG81317-CH|
|Rat CLDN11 ORF mammalian expression plasmid, C-Myc tag||RG81317-CM|
|Rat CLDN11 ORF mammalian expression plasmid, C-HA tag||RG81317-CY|
|Rat CLDN11 Gene cDNA clone plasmid||RG81317-G|
|Rat CLDN11 ORF mammalian expression plasmid, N-Flag tag||RG81317-NF|
|Rat CLDN11 ORF mammalian expression plasmid, N-His tag||RG81317-NH|
|Rat CLDN11 ORF mammalian expression plasmid, N-Myc tag||RG81317-NM|
|Rat CLDN11 ORF mammalian expression plasmid, N-HA tag||RG81317-NY|
|Rat CLDN11 natural ORF mammalian expression plasmid||RG81317-UT|
|Learn more about expression Vectors|
Claudin-11, also known as CLDN11, belongs to the group of claudins. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands function as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions.Claudin-11 is a tight junction associated protein and is a major component of central nervous system (CNS) myelin that is necessary for normal CNS function. Human blood-testis barrier disruption is related to a dysfunction of CLDN11 gene. It plays an important role in regulating proliferation and migration of oligodendrocytes.