|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat ARG1 ORF mammalian expression plasmid, C-GFPSpark tag||RG81309-ACG|
|Rat ARG1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81309-ACR|
|Rat ARG1 ORF mammalian expression plasmid, N-GFPSpark tag||RG81309-ANG|
|Rat ARG1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG81309-ANR|
|Rat ARG1 ORF mammalian expression plasmid, C-Flag tag||RG81309-CF|
|Rat ARG1 ORF mammalian expression plasmid, C-His tag||RG81309-CH|
|Rat ARG1 ORF mammalian expression plasmid, C-Myc tag||RG81309-CM|
|Rat ARG1 ORF mammalian expression plasmid, C-HA tag||RG81309-CY|
|Rat ARG1 ORF mammalian expression plasmid, N-Flag tag||RG81309-NF|
|Rat ARG1 ORF mammalian expression plasmid, N-His tag||RG81309-NH|
|Rat ARG1 ORF mammalian expression plasmid, N-Myc tag||RG81309-NM|
|Rat ARG1 ORF mammalian expression plasmid, N-HA tag||RG81309-NY|
|Rat ARG1 Gene cDNA clone plasmid||RG81309-U|
|Rat ARG1 natural ORF mammalian expression plasmid||RG81309-UT|
|Learn more about expression Vectors|
Arginase is the focal enzyme of the urea cycle hydrolysing L-arginine to urea and L-ornithine. Emerging studies have identified arginase in the vasculature and have implicated this enzyme in the regulation of nitric oxide (NO) synthesis and the development of vascular disease. Arginase also redirects the metabolism of L-arginine to L-ornithine and the formation of polyamines and L-proline, which are essential for smooth muscle cell growth and collagen synthesis. Arginase is encoded by two recently discovered genes (Arginase I and Arginase II). In most mammals, Arginase 1 (ARG1) also known as Arginase, liver, which functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. It may be found at lower levels in macrophages, lactating mammary glands, and brain.