|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse IL1RAP ORF mammalian expression plasmid, C-GFPSpark tag||MG52657-ACG|
|Mouse IL1RAP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52657-ACR|
|Mouse IL1RAP ORF mammalian expression plasmid, C-Flag tag||MG52657-CF|
|Mouse IL1RAP ORF mammalian expression plasmid, C-His tag||MG52657-CH|
|Mouse IL1RAP ORF mammalian expression plasmid, C-Myc tag||MG52657-CM|
|Mouse IL1RAP ORF mammalian expression plasmid, C-HA tag||MG52657-CY|
|Mouse IL1RAP Gene cDNA clone plasmid||MG52657-G|
|Mouse IL1RAP ORF mammalian expression plasmid, N-Flag tag||MG52657-NF|
|Mouse IL1RAP ORF mammalian expression plasmid, N-His tag||MG52657-NH|
|Mouse IL1RAP ORF mammalian expression plasmid, N-Myc tag||MG52657-NM|
|Mouse IL1RAP ORF mammalian expression plasmid, N-HA tag||MG52657-NY|
|Mouse IL1RAP natural ORF mammalian expression plasmid||MG52657-UT|
|Learn more about expression Vectors|
Interleukin-1 receptor accessory protein (IL-1RAcP) also known as Interleukin-1 receptor member 3 (IL-1R3) is a a cytokine receptor which binds interleukin 1. The IL-1 receptor accessory protein (IL1RAP) is a transmembrane protein that interacts with IL-1R and is required for IL-1 signal transduction. Interleukin 1 induces synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. IL-1RAcP/IL-1R3 is a necessary part of the interleukin 1 receptor complex which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. IL-1RAcP/IL-1R3 mediates interleukin-1-dependent activation of NF-kappa-B. Isoform 1 is part of the membrane-bound form of the IL-1 receptor. Signaling involves formation of a ternary complex containing IL1R1, TOLLIP, MYD88, and IRAK1 or IRAK2. Isoform 2 modulates the response to interleukins by associating with soluble IL1R1 and enhancing interleukin-binding to the decoy receptor.