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Mouse PDGFB ORF mammalian expression plasmid, N-His tag

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Mouse PDGFB cDNA Clone Product Information
Gene_bank_ref_id:NM_011057.3
RefSeq ORF Size:726bp
cDNA Description:Full length Clone DNA of Mus musculus platelet derived growth factor, B polypeptide with N terminal His tag.
Gene Synonym:Sis, PDGF-B, Pdgfb
Species:Mouse
Vector:pCMV3-SP-N-His
Plasmid:pCMV3-His-mPDGFB
Restriction Site:KpnI + XbaI (6kb + 0.76kb)
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 354T/G not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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Catalog: MG50801-NH
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