|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse ILKAP ORF mammalian expression plasmid, C-GFPSpark tag||MG52651-ACG|
|Mouse ILKAP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52651-ACR|
|Mouse ILKAP ORF mammalian expression plasmid, N-GFPSpark tag||MG52651-ANG|
|Mouse ILKAP ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52651-ANR|
|Mouse ILKAP ORF mammalian expression plasmid, C-Flag tag||MG52651-CF|
|Mouse ILKAP ORF mammalian expression plasmid, C-His tag||MG52651-CH|
|Mouse ILKAP ORF mammalian expression plasmid, C-Myc tag||MG52651-CM|
|Mouse ILKAP ORF mammalian expression plasmid, C-HA tag||MG52651-CY|
|Mouse ILKAP Gene cDNA clone plasmid||MG52651-G|
|Mouse ILKAP ORF mammalian expression plasmid, N-Flag tag||MG52651-NF|
|Mouse ILKAP ORF mammalian expression plasmid, N-His tag||MG52651-NH|
|Mouse ILKAP ORF mammalian expression plasmid, N-Myc tag||MG52651-NM|
|Mouse ILKAP ORF mammalian expression plasmid, N-HA tag||MG52651-NY|
|Mouse ILKAP natural ORF mammalian expression plasmid||MG52651-UT|
|Learn more about expression Vectors|
Integrin-linked kinase-associated serine/threonine phosphatase 2C, also known as ILKAP, is cytoplasm protein which belongs to the PP2C family. ILKAP contains one PP2C-like domain. ILKAP is widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues. ILKAP may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. ILKAP selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. ILKAP inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation. Integrin-linked kinase ( ILK ) plays key roles in a variety of cell functions, including cell proliferation, adhesion and migration. Within the cell, ILK localizes to multiple sites, including the cytoplasm, focal adhesion complexes that mediate cell adhesion to extracellular substrates, as well as cell-cell junctions in epidermal keratinocytes. Nuclear ILK can be rapidly exported into the cytoplasm through a CRM1-dependent pathway, and its export is enhanced by the type 2C protein phosphatase ILKAP. Nuclear localization of ILK in epidermal keratinocytes is associated with increased DNA synthesis, which is sensitive to inhibition by ILKAP.