|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CTSE ORF mammalian expression plasmid, C-GFPSpark tag||RG81240-ACG|
|Rat CTSE ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81240-ACR|
|Rat CTSE ORF mammalian expression plasmid, C-Flag tag||RG81240-CF|
|Rat CTSE ORF mammalian expression plasmid, C-His tag||RG81240-CH|
|Rat CTSE ORF mammalian expression plasmid, C-Myc tag||RG81240-CM|
|Rat CTSE ORF mammalian expression plasmid, C-HA tag||RG81240-CY|
|Rat CTSE ORF mammalian expression plasmid, N-Flag tag||RG81240-NF|
|Rat CTSE ORF mammalian expression plasmid, N-His tag||RG81240-NH|
|Rat CTSE ORF mammalian expression plasmid, N-Myc tag||RG81240-NM|
|Rat CTSE ORF mammalian expression plasmid, N-HA tag||RG81240-NY|
|Rat CTSE Gene cDNA clone plasmid||RG81240-U|
|Rat CTSE natural ORF mammalian expression plasmid||RG81240-UT|
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Cathepsin E Protein (CTSE Protein) is a member of the peptidase C1 family that is a gastric aspartic protease that functions as a disulfide-linked homodimer. Cathepsin E Protein (CTSE Protein) is predominantly present in the cells of immune system and is frequently implicated in antigen processing via the MHC classⅡ pathway which however does not appear to be involved in the digestion of dietary protein. The protein has a specificity similar to that of pepsin and pepsin. Cathepsin E Protein (CTSE Protein) is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach and also been found in more than half of the gastric cancers. It appears, therefore, to be an oncofetal antigen.