|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat KNG1 ORF mammalian expression plasmid, C-GFPSpark tag||RG81182-ACG|
|Rat KNG1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81182-ACR|
|Rat KNG1 ORF mammalian expression plasmid, C-Flag tag||RG81182-CF|
|Rat KNG1 ORF mammalian expression plasmid, C-His tag||RG81182-CH|
|Rat KNG1 ORF mammalian expression plasmid, C-Myc tag||RG81182-CM|
|Rat KNG1 ORF mammalian expression plasmid, C-HA tag||RG81182-CY|
|Rat KNG1 ORF mammalian expression plasmid, N-Flag tag||RG81182-NF|
|Rat KNG1 ORF mammalian expression plasmid, N-His tag||RG81182-NH|
|Rat KNG1 ORF mammalian expression plasmid, N-Myc tag||RG81182-NM|
|Rat KNG1 ORF mammalian expression plasmid, N-HA tag||RG81182-NY|
|Rat KNG1 Gene cDNA clone plasmid||RG81182-U|
|Rat KNG1 natural ORF mammalian expression plasmid||RG81182-UT|
|Learn more about expression Vectors|
Mouse kininogen-1, also known as high molecular weight kininogen, williams-Fitzgerald-Flaujeac factor, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, KNG1 and BDK, is a secreted protein which contains three cystatin domains. Kininogen-1 / KNG1 is a protein from the blood coagulation system as well as the kinin-kallikrein system. It is a protein that adsorbs to the surface of biomaterials that come in contact with blood. Kininogen-1 / KNG1 circulates throughout the blood and quickly adsorbs to the material surfaces. Kininogen-1 / KNG1 is one of the early participants of the intrinsic pathway of coagulation, together with Factor XII (Hageman factor) and prekallikrein. Kininogen-1 / KNG1 is one of the kininogens, a class of proteins. As with many other coagulation proteins, the protein was initially named after the patients in whom deficiency was first observed. When the clinical data were combined, it turned out that all patients, in fact, had a deficiency of the same protein. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) which is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.