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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rhesus RBBP4 ORF mammalian expression plasmid, C-GFPSpark tag||CG90743-ACG|
|Rhesus RBBP4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90743-ACR|
|Rhesus RBBP4 ORF mammalian expression plasmid, N-GFPSpark tag||CG90743-ANG|
|Rhesus RBBP4 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||CG90743-ANR|
|Rhesus RBBP4 ORF mammalian expression plasmid, C-Flag tag||CG90743-CF|
|Rhesus RBBP4 ORF mammalian expression plasmid, C-His tag||CG90743-CH|
|Rhesus RBBP4 ORF mammalian expression plasmid, C-Myc tag||CG90743-CM|
|Rhesus RBBP4 ORF mammalian expression plasmid, C-HA tag||CG90743-CY|
|Rhesus RBBP4 Gene cDNA clone plasmid||CG90743-G|
|Rhesus RBBP4 ORF mammalian expression plasmid, N-Flag tag||CG90743-NF|
|Rhesus RBBP4 ORF mammalian expression plasmid, N-His tag||CG90743-NH|
|Rhesus RBBP4 ORF mammalian expression plasmid, N-Myc tag||CG90743-NM|
|Rhesus RBBP4 ORF mammalian expression plasmid, N-HA tag||CG90743-NY|
|Rhesus RBBP4 natural ORF mammalian expression plasmid||CG90743-UT|
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Histone-binding protein RBBP4, also known as Retinoblastoma-binding protein 4, Retinoblastoma-binding protein p48, Chromatin assembly factor 1 subunit C, Chromatin assembly factor I p48 subunit, Nucleosome-remodeling factor subunit RBAP48 and RBBP4, is a nucleus protein which belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. RBBP4 is a component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling and the NURF (nucleosome remodeling factor) complex.
One common myth is that age-related memory loss is an early indication of Alzheimer's disease. But researchers at the Columbia University Medical Center in New York City have found a specific protein, RbAp48, that they believe is responsible for age-related memory problems. What's more, by replenishing RbAp48 in the brains of mice, the researchers were able to undo existing age-related memory damage.
To find RbAp48, researchers focused on the hippocampus, the region of the brain where memories are formed. After studying eight healthy brains donated to science by people between the ages of 33 and 88, they found that RbAp48 was reduced by nearly 50 percent in the older brains. The researchers found that when they turned off RbAp48 in younger mice, they became more forgetful, while increasing RbAp48 in older mice restored memory. The mice were given memory tests that included object recognition and water maze problems.