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Human CTLA4 / CD152 Protein (His Tag)

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Human CTLA4 Protein Product Information
Synonym:CTLA4, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, ICOS, IDDM12
Protein Construction:A DNA sequence encoding the human CTLA4 (NP_005205.2) extracellular domain (Met 1-Phe 162) was fused with the a polyhistidine tag at the C-terminus.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human CTLA4 Protein QC Testing
Purity:> 90 % as determined by SDS-PAGE
Bio-Activity:1. Measured by its binding ability in a functional ELISA.
2. Immobilized human CTLA4 (cat: 11159-H08H) at 10 μg/mL (100 μl/well) can bind human B7-1/Fc (cat: 10698-H03H), The EC50 of human B7-1/Fc (cat: 10698-H03H is 14 ng/mL.
3. Immobilized human CTLA4 (cat: 11159-H08H) at 10 μg/mL (100 μl/well) can bind human B7-2/Fch (cat: 10699-H03H), The EC50 of human B7-2/Fch (cat: 10699-H03H) is 53 ng/mL.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Ala 37
Molecule Mass:The recombinant human CTLA4 consists of 137 amino acids and has a predicted molecular mass of 15 kDa.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human CTLA4 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other CTLA4 Recombinant Protein Products
CTLA-4/CD152 Background

Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).

Human CTLA-4/CD152 References
  • Slavik JM, et al. (1999) CD28/CTLA-4 and CD80/CD86 families: signaling and function. Immunol Res. 19(1): 1-24.
  • Holmberg D, et al. (2005) CTLA-4 (CD152) and its involvement in autoimmune disease. Autoimmunity. 38(3): 225-33.
  • Chin LT, et al. (2008) Immune intervention with monoclonal antibodies targeting CD152 (CTLA-4) for autoimmune and malignant diseases. Chang Gung Med J. 31(1): 1-15.
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    Catalog: 11159-H08H-100
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