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Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone)

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DIABLOcDNA Clone Product Information
Gene_bank_ref_id:XM_005572484.1
cDNA Size:561
cDNA Description:ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) diablo, IAP-binding mitochondrial protein DNA.
Gene Synonym:DIABLO
Species:Cynomolgus
Vector:pGEM-T Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence. Please check the sequence information before order.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone) on other vectors
Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedCG90706-ACG$325
Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagCG90706-ACR$325
Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedCG90706-ANG$325
Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagCG90706-ANR$325
Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedCG90706-CF$295
Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedCG90706-CH$295
Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedCG90706-CM$295
Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedCG90706-CY$295
Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone)CG90706-G$95
Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedCG90706-NF$295
Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedCG90706-NH$295
Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedCG90706-NM$295
Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedCG90706-NY$295
Cynomolgus monkey DIABLO Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedCG90706-UT$295
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Background

Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer.

References
  • Korga A, et al. (2006) Role of mitochondrial protein Smac/Diablo in regulation of apoptotic pathways Pol Merkur Lekarski. 20(119): 573-6.
  • Anguiano-Hernandez YM, et al. (2007) Smac/DIABLO and colon cancer. Anticancer Agents Med Chem. 7(4): 467-73.
  • Martinez-Ruiz G, et al. (2008) Role of Smac/DIABLO in cancer progression. J Exp Clin Cancer Res. 27: 48.
  • Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"