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Human PFN4 ORF mammalian expression plasmid, N-HA tag

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Human PFN4 cDNA Clone Product Information
Gene_bank_ref_id:BC029523
RefSeq ORF Size:390bp
cDNA Description:Full length Clone DNA of Homo sapiens profilin family, member 4 with N terminal HA tag.
Gene Synonym:PFN4
Species:Human
Vector:pCMV3-N-HA
Plasmid:
Restriction Site:
Tag Sequence:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Sequence Description:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

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Background

PFN4, also known as profilin 4, is a member of the profilin family. Profilin can be detected in all eukaryotic organisms. It plays an important role in the spatially and temporally controlled growth of actin microfilaments. Profilin is one of the most abundant actin monomer binders, but proteins such as CAP and (in mammals) thymosin β4 have some functional overlaps with profilin. In contrast, ADF/cofilin has some properties that antagonize profilin action. PFN4 also functions in the dynamic turnover and restructuring of the actin cytoskeleton.

References
  • Di Nardo A. et al., 2000, J Cell Sci. 113 (21): 3795-803.
  • Witke W. et al., 1998, EMBO J. 17 (4): 967-76.
  • Carlsson L. et al., 1977, J Mol Biol. 115 (3): 465-83.
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    Catalog: HG15093-NY
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"