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Human SMPD1 / ASM Protein (His Tag)

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Human SMPD1/ASM Protein Product Information
Synonym:ASM, NPD, SMPD1
Protein Construction:A DNA sequence encoding the mature form of human SMPD1 isoform 1 (NP_000534.3) (Gly 85-Cys 631) was expressed, fused with a polyhistidine tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human SMPD1/ASM Protein QC Testing
Purity:> 91 % as determined by SDS-PAGE
Bio-Activity:Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC) . The specific activity is 77 pmol/min/μg.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:His
Molecule Mass:The secreted recombinant human SMPD1 consists of 563 amino acids and predicts a molecular mass of 65 kDa as estimated by SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human SMPD1/ASM Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human SMPD1/ASM Protein SDS-PAGE
Human SMPD1 / ASM Protein (His Tag) SDS-PAGE
Other SMPD1/ASM Recombinant Protein Products
SMPD1 Background

Sphingomyelin phosphodiesterase 1 (SMPD1) , also known as ASM ( acid sphingomyelinase ), is a member of the acid sphingomyelinase family of enzymes. Three isoforms have been identified, isoform 1 is 631 amino acids (aa) in length as the pro form, while Isoform 2 and isoform 3 have lost catalytic activity. The active SMPD1 isoform 1 contains one saposin B-type domain that likely interacts with sphingomyelin, and a catalytic region. Human SMPD1 is 86% aa identical to mouse SMPD1. SMPD1 is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid ) into ceramide through the removal of phosphorylcholine. This generates second messenger components that participate in signal transduction. Defects in SMPD1 are the cause of Niemann-Pick disease type A (NPA) and type B (NPB), also known as Niemann-Pick disease classical infantile form and Niemann-Pick disease visceral form. Niemann-Pick disease is a clinically and genetically heterogeneous recessive disorder. NPB has little if any neurologic involvement and patients may survive into adulthood.

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Catalog: 11087-H07B-10
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