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Mouse SPON1 ORF mammalian expression plasmid, N-His tag

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Mouse SPON1 cDNA Clone Product Information
NCBI RefSeq:NM_145584.2
RefSeq ORF Size:2424bp
cDNA Description:Full length Clone DNA of Mus musculus spondin 1, (f-spondin) extracellular matrix protein with N terminal His tag.
Gene Synonym:AI666765, AW455831, BC020531, D330035F22Rik, Spon1
Species:Mouse
Vector:pCMV3-SP-N-His
Plasmid:
Restriction Site:
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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Catalog: MG50604-NH
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