|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat APOA1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80793-ACG|
|Rat APOA1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80793-ACR|
|Rat APOA1 ORF mammalian expression plasmid, C-Flag tag||RG80793-CF|
|Rat APOA1 ORF mammalian expression plasmid, C-His tag||RG80793-CH|
|Rat APOA1 ORF mammalian expression plasmid, C-Myc tag||RG80793-CM|
|Rat APOA1 ORF mammalian expression plasmid, C-HA tag||RG80793-CY|
|Rat APOA1 ORF mammalian expression plasmid, N-Flag tag||RG80793-NF|
|Rat APOA1 ORF mammalian expression plasmid, N-His tag||RG80793-NH|
|Rat APOA1 ORF mammalian expression plasmid, N-Myc tag||RG80793-NM|
|Rat APOA1 ORF mammalian expression plasmid, N-HA tag||RG80793-NY|
|Rat APOA1 Gene cDNA clone plasmid||RG80793-U|
|Rat APOA1 natural ORF mammalian expression plasmid||RG80793-UT|
|Learn more about expression Vectors|
Apolipoprotein A1 (APOA1) is a member of the apolipoprotein family whose members are proteins bind with lipids and form lipoproteins to translate these oil-soluble lipids such as fat and cholesterol through lymphatic and circulatory system. APOA1 is the main component of high density lipoprotein (HDL) in plasma and is involved in the esterification of cholesterol as a cofactor of lecithin-cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters, and thus play a major role in cholesterol efflux from peripheral cells. As a major component of the HDL complex, APOA1 helps to clear cholesterol from arteries. APOA1 is also characterized as a prostacyclin stabilizing factor, and thus may have an anticlotting effect. Defects in encoding gene may result in HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Men carrying a mutation may develop premature coronary artery disease.