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Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA Antibody, Mouse MAb

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Influenza HA Antibody Product Information
Immunogen:Recombinant H1N1 HA protein
Clone ID:9G1G8
Ig Type:Mouse IgG2b
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza A virus H1N1 hemagglutinin (HA) extracellular domain. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Influenza HA Antibody Usage Guide
Specificity:H1N1 (A/California/04/2009) HA
H1N1 (A/California/07/2009) HA
Has cross-reactivity in ELISA with
H1N2 (A/swine/Guangxi/13/2006) HA
H1N3 (A/duck/NZL/160/1976) HA
H5N1 (A/Anhui/1/2005) HA
H5N1 (A/Viet nam/1194/2004) HA
H5N1 (A/Indonesia/5/2005) HA
H5N1 (A/turkey/Turkey/1/2005) HA
H5N1 (A/bar-headed goose/Qinghai/14/2008) HA
No cross-reactivity in WB and ELISA with
H1N1 (A/Brisbane/59/2007) HA
H1N1 (A/BrevigMission/1/1918) HA
H1N1 (A/Solomon Islands/3/2006) HA
H1N1 (A/Ohio/UR06-0091/2007) HA
H1N1 (A/New Caledonia/20/1999) HA
H1N1 (A/Puerto Rico/8/1934) HA
H1N1 (A/WSN/1933) HA
H3N2 (A/Brisbane/10/2007) HA
Influenza B (B/Florida/4/2006) HA
Human cell lysate (293 cell line)
Application:WB, ELISA, FCM

WB: 1-2 μg/mL

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect H1N1 HA. The detection limit for H1N1 HA is approximately 10 ng/well.

FCM: 0.5-2 μg/Test

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Influenza HA Antibody FC Application Image
[Click to enlarge image]
Caption:
High Five cells were infected by Bac-HA, cells were collected at 48 hours postinfection, and the cell surface expression of HA were measured by flow cytometry. 106 Cells were stained with 1 μg Purified Mouse Anti-H1N1-HA (11055-MM08) antibody for 20 min on ice. Cells were washed twice and incubated with 1 μg of a FITC Goat Anti-Mouse Ig secondary antibody for 20 min on ice. Cells were washed twice and analyzed by flow cytometry.
Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA Antibody, Mouse MAb, Flow cytometric
[Click to enlarge image]
Caption:
High Five cells were infected by Bac-HA, cells were collected at 48 hours postinfection, and the cell surface expression of HA were measured by flow cytometry. 106 Cells were stained with 1 μg Purified Mouse Anti-H1N1-HA (11055-MM08) antibody for 20 min on ice. Cells were washed twice and incubated with 1 μg of a FITC Goat Anti-Mouse Ig secondary antibody for 20 min on ice. Cells were washed twice and analyzed by flow cytometry.
Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA Antibody, Mouse MAb, Flow cytometric
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All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"