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Cynomolgus monkey ACO2 Gene cDNA clone plasmid

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Cynomolgus ACO2 cDNA Clone Product Information
Gene_bank_ref_id:unsubmitted
RefSeq ORF Size:2343bp
cDNA Description:Full length Clone DNA of Macaca mulatta aconitase 2, mitochondrial.
Gene Synonym:ACO2
Species:Cynomolgus
Vector:pGEM-T Vector
Plasmid:pGEM-cynoACO2
Restriction Site:
Tag Sequence:
Sequence Description:Identical with NM_001261164.2 [ Macaca mulatta (Rhesus monkey) ]: 501G/A, 1446A/G and 1569G/A not causing the amino acid variation. Please check the sequence information before order.
Sequencing primers:
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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References
  • Robbins AH, et al. (1989) The structure of aconitase. Proteins. 5 (4): 289-312.
  • Lauble H, et al. (1992) Crystal structures of aconitase with isocitrate and nitroisocitrate bound. Biochemistry. 31 (10): 2735-48.
  • Robbins AH, et al. (1989) Structure of activated aconitase: formation of the 4Fe-4S cluster in the crystal. Proc Natl Acad Sci. 86 (10): 3639-43.
  • Size / Price
    Catalog: CG90615-G
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"