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Human PHKG1 Protein (GST Tag)

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Human PHKG1 Protein Product Information
Synonym:PHKG, PHKG1
Protein Construction:A DNA sequence encoding the human PHKG1 (NP_006204.1) (Met 1-Tyr 387) was fused with the GST tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human PHKG1 Protein QC Testing
Purity:> 87 % as determined by SDS-PAGE
Bio-Activity:No Kinase Activity
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human PHKG1/GST chimera consists of 612 amino acids and predicts a molecular mass of 71.3 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM GSH, 0.5mM PMSF, pH 8.0
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human PHKG1 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human PHKG1 Protein SDS-PAGE
Human PHKG1 Protein (GST Tag) SDS-PAGE
Human PHKG1 Protein (GST Tag) SDS-PAGE
Other PHKG1 Recombinant Protein Products
PHKG1 Background

Phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform, also known as Phosphorylase kinase subunit gamma-1 and PHKG1, is a member of the protein kinase superfamily and CAMK Ser/Thr protein kinase family. PHKG1 is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. Muscle glycogenosis caused by phosphorylase kinase (Phk) deficiency may lead to exercise intolerance, weakness and musculatur atrophy. The gene encoding the muscle isoform of the Phk gamma subunit (gamma M) is one of the candidate genes in which mutations responsible for this condition should be sought. Muscle-specific deficiency of Phk causes glycogen storage disease, clinically manifesting in exercise intolerance with early fatiguability, pain, cramps and occasionally myoglobinuria. 

Human PHKG1 References
  • Wehner, M. et al., 1995, Hum Genet. 96 (5): 616-8.
  • Burwinkel, B. et al., 2003, Eur J Hum Genet. 11 (7): 516-26.
  • Winchester, JS. et al., 2007, Mol Genet Metab. 92 (3): 234-42.
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    Catalog: 11034-H09B-20
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