|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat PRDX2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80710-ACG|
|Rat PRDX2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80710-ACR|
|Rat PRDX2 ORF mammalian expression plasmid, N-GFPSpark tag||RG80710-ANG|
|Rat PRDX2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80710-ANR|
|Rat PRDX2 ORF mammalian expression plasmid, C-Flag tag||RG80710-CF|
|Rat PRDX2 ORF mammalian expression plasmid, C-His tag||RG80710-CH|
|Rat PRDX2 ORF mammalian expression plasmid, C-Myc tag||RG80710-CM|
|Rat PRDX2 ORF mammalian expression plasmid, C-HA tag||RG80710-CY|
|Rat PRDX2 ORF mammalian expression plasmid, N-Flag tag||RG80710-NF|
|Rat PRDX2 ORF mammalian expression plasmid, N-His tag||RG80710-NH|
|Rat PRDX2 ORF mammalian expression plasmid, N-Myc tag||RG80710-NM|
|Rat PRDX2 ORF mammalian expression plasmid, N-HA tag||RG80710-NY|
|Rat PRDX2 Gene cDNA clone plasmid||RG80710-U|
|Rat PRDX2 natural ORF mammalian expression plasmid||RG80710-UT|
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Peroxiredoxin-2, also known as Natural killer cell-enhancing factor B, NKEF-B, Thiol-specific antioxidant protein, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, PRDX2 and NKEFB, is a cytoplasm protein which belongs to the ahpC / TSA family. Peroxiredoxin-2 / PRDX2 contains one thioredoxin domain. Peroxiredoxin-2 / PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. Peroxiredoxin-2 / PRDX2 is not able to receive electrons from glutaredoxin. It may play an important role in eliminating peroxides generated during metabolism. Peroxiredoxin-2 / PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2.
The Peroxiredoxins / Prx are a family of peroxidases that can reduce H2O2 using an electron from thioredoxin (Trx) or other substances. The mammalian Peroxiredoxins / Prx family is divided into six groups ( PRDX1，PRDX2, PRDX3, PRDX4, PRDX5, PRDX6 ) on the basis of homology of amino acid sequences. They are located in the cytosol and play a role in the cell signaling system. All six mammalian peroxiredoxins are expressed in the lung. Peroxiredoxins / Prx is overexpressed in breast cancer tissues to a great extent suggesting that Peroxiredoxins / Prx has a proliferative effect and may be related to cancer development or progression.