|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat ECH1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80709-ACG|
|Rat ECH1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80709-ACR|
|Rat ECH1 ORF mammalian expression plasmid, N-GFPSpark tag||RG80709-ANG|
|Rat ECH1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80709-ANR|
|Rat ECH1 ORF mammalian expression plasmid, C-Flag tag||RG80709-CF|
|Rat ECH1 ORF mammalian expression plasmid, C-His tag||RG80709-CH|
|Rat ECH1 ORF mammalian expression plasmid, C-Myc tag||RG80709-CM|
|Rat ECH1 ORF mammalian expression plasmid, C-HA tag||RG80709-CY|
|Rat ECH1 ORF mammalian expression plasmid, N-Flag tag||RG80709-NF|
|Rat ECH1 ORF mammalian expression plasmid, N-His tag||RG80709-NH|
|Rat ECH1 ORF mammalian expression plasmid, N-Myc tag||RG80709-NM|
|Rat ECH1 ORF mammalian expression plasmid, N-HA tag||RG80709-NY|
|Rat ECH1 Gene cDNA clone plasmid||RG80709-U|
|Rat ECH1 natural ORF mammalian expression plasmid||RG80709-UT|
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ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.