|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat RAMP3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80690-ACG|
|Rat RAMP3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80690-ACR|
|Rat RAMP3 ORF mammalian expression plasmid, C-Flag tag||RG80690-CF|
|Rat RAMP3 ORF mammalian expression plasmid, C-His tag||RG80690-CH|
|Rat RAMP3 ORF mammalian expression plasmid, C-Myc tag||RG80690-CM|
|Rat RAMP3 ORF mammalian expression plasmid, C-HA tag||RG80690-CY|
|Rat RAMP3 ORF mammalian expression plasmid, N-Flag tag||RG80690-NF|
|Rat RAMP3 ORF mammalian expression plasmid, N-His tag||RG80690-NH|
|Rat RAMP3 ORF mammalian expression plasmid, N-Myc tag||RG80690-NM|
|Rat RAMP3 ORF mammalian expression plasmid, N-HA tag||RG80690-NY|
|Rat RAMP3 Gene cDNA clone plasmid||RG80690-U|
|Rat RAMP3 natural ORF mammalian expression plasmid||RG80690-UT|
|Learn more about expression Vectors|
RAMP3 belongs to the RAMP family. Members of this family are single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs have a wide biological distribution; high concentrations are found in the brain, lung, liver, heart and spleen with lower expression levels present in the testes, gastrointestinal tract and thyroid. RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. They are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of RAMP3 protein, CRLR functions as an adrenomedullin receptor.