|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat GAPDH ORF mammalian expression plasmid, C-GFPSpark tag||RG80680-ACG|
|Rat GAPDH ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80680-ACR|
|Rat GAPDH ORF mammalian expression plasmid, N-GFPSpark tag||RG80680-ANG|
|Rat GAPDH ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80680-ANR|
|Rat GAPDH ORF mammalian expression plasmid, C-Flag tag||RG80680-CF|
|Rat GAPDH ORF mammalian expression plasmid, C-His tag||RG80680-CH|
|Rat GAPDH ORF mammalian expression plasmid, C-Myc tag||RG80680-CM|
|Rat GAPDH ORF mammalian expression plasmid, C-HA tag||RG80680-CY|
|Rat GAPDH ORF mammalian expression plasmid, N-Flag tag||RG80680-NF|
|Rat GAPDH ORF mammalian expression plasmid, N-His tag||RG80680-NH|
|Rat GAPDH ORF mammalian expression plasmid, N-Myc tag||RG80680-NM|
|Rat GAPDH ORF mammalian expression plasmid, N-HA tag||RG80680-NY|
|Rat GAPDH Gene cDNA clone plasmid||RG80680-U|
|Rat GAPDH natural ORF mammalian expression plasmid||RG80680-UT|
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Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.