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Transferrin Receptor / TFRC / CD71 Antibody, Rabbit PAb, Antigen Affinity Purified

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Human TFRC Antibody Product Information
Immunogen:Recombinant Human Transferrin Receptor / TFRC / CD71 protein (Catalog#11020-H07H)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human Transferrin Receptor / TFRC / CD71 (rh Transferrin Receptor / TFRC / CD71; Catalog#11020-H07H; NP_003225.2; Cys89-Phe 760). Transferrin Receptor / TFRC / CD71 specific IgG was purified by Human Transferrin Receptor / TFRC / CD71 affinity chromatography.
Human TFRC Antibody Usage Guide
Specificity:Human Transferrin Receptor / TFRC / CD71
Application:WB, ELISA, IHC-P, IP

WB: 1-2 μg/mL

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human TFRC. The detection limit for Human TFRC is approximately 0.00245 ng/well.

IHC-P: 0.1-2 μg/mL

IP: 1-4 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human TFRC Antibody WB Application Image
Transferrin Receptor / TFRC / CD71 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Transferrin Receptor / TFRC / CD71 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Human TFRC Antibody IHC Application Image
[Click to enlarge image]
Caption:
Immunochemical staining of human TFRC in human colon carcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to intestinal gland.
Transferrin Receptor / TFRC / CD71 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human TFRC in human colon carcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to intestinal gland.
Transferrin Receptor / TFRC / CD71 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human TFRC in human liver with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.
Transferrin Receptor / TFRC / CD71 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human TFRC in human liver with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.
Transferrin Receptor / TFRC / CD71 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human TFRC in human placenta with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.
Transferrin Receptor / TFRC / CD71 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human TFRC in human placenta with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.
Transferrin Receptor / TFRC / CD71 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Human TFRC Antibody IP Application Image
Transferrin Receptor / TFRC / CD71 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
Other TFRC Antibody Products
Transferrin Receptor/TFRC/CD71 Background

Mouse transferrin receptor protein 1, also known as transferrin receptor, Trfr, p90, CD71 and TFRC, is a single-pass type II membrane protein which belongs to the peptidase M28 family and M28B subfamily. TFRC / CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a diagnostic tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC / CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. TFRC / CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential diagnostic and therapeutic target.

Human Transferrin Receptor/TFRC/CD71 References
  • Douabin-Gicquel V., et al., 2001,Hum. Genet. 109:393-401.
  • Ryschich,E. et al., 2004,Eur J Cancer. 40 (9):1418-22.
  • Tosoni D., et al., 2005, Cell 123:875-888.
  • Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"