|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat NRAS ORF mammalian expression plasmid, C-GFPSpark tag||RG80678-ACG|
|Rat NRAS ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80678-ACR|
|Rat NRAS ORF mammalian expression plasmid, C-Flag tag||RG80678-CF|
|Rat NRAS ORF mammalian expression plasmid, C-His tag||RG80678-CH|
|Rat NRAS ORF mammalian expression plasmid, C-Myc tag||RG80678-CM|
|Rat NRAS ORF mammalian expression plasmid, C-HA tag||RG80678-CY|
|Rat NRAS ORF mammalian expression plasmid, N-Flag tag||RG80678-NF|
|Rat NRAS ORF mammalian expression plasmid, N-His tag||RG80678-NH|
|Rat NRAS ORF mammalian expression plasmid, N-Myc tag||RG80678-NM|
|Rat NRAS ORF mammalian expression plasmid, N-HA tag||RG80678-NY|
|Rat NRAS Gene cDNA clone plasmid||RG80678-U|
|Rat NRAS natural ORF mammalian expression plasmid||RG80678-UT|
|Learn more about expression Vectors|
NRAS was discovered by researchers at the Institute of Cancer Research, funded by the Cancer Research Campaign (now Cancer Research UK). NRAS gene is a member of the Ras gene family. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. The mammalian ras gene family consists of the harvey and kirsten ras genes (HRAS and KRAS), an inactive pseudogene of each (c-Hras2 and c-Kras1) and the N-ras gene. They differ significantly only in the C-terminal 40 amino acids. These ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. The NRAS gene specifies two main transcripts of 2Kb and 4.3Kb. The difference between the two transcripts is a simple extension through the termination site of the 2Kb transcript. The NRAS gene consists of seven exons (-I, I, II, III, IV, V, VI).