Quick Order

Human Hexokinase-3 / HK3 Protein (His & GST Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Human HK3 Protein Product Information
Synonym:IMP-2, IMP2, VICKZ2, p62
Protein Construction:A DNA sequence encoding the human HK3 (P52790) (Met 1-Val 923) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Human HK3 Protein QC Testing
Purity:> 93 % as determined by SDS-PAGE
Bio-Activity:Kinase activity untested
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human HK3/GST chimera consists of 1160 amino acids and has a calculated molecular mass of 127 kDa. It migrates as an approximately 115 kDa band in SDS-PAGE under reducing conditions.
Formulation:Supplied as sterile 50mM Tris, 100mM NaCl, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human HK3 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human HK3 Protein SDS-PAGE
Human Hexokinase-3 / HK3 Protein (His & GST Tag) SDS-PAGE
Human Hexokinase-3 / HK3 Protein (His & GST Tag) SDS-PAGE
Other HK3 Recombinant Protein Products
Hexokinase 3 Background

Hexokinase-3, also known as Hexokinase type III, HKIII and HK3, is a protein which belongs to the hexokinase family. Hexokinase-3 / HK3 is an enzyme which in humans is encoded by the HK2 gene. Hexokinases phosphorylate glucose to produce glucose 6-phosphate, committing glucose to the glycolytic pathway. In mammalian tissues hexokinase exists as four isoenzymes encoded by distinct genes. These proteins are homologous and are organized in two homologous domains, with the exception of hexokinase type IV which has only one. This organization is believed to be the result of a duplication and tandem fusion event involving the gene encoding for the ancestral hexokinase. The gene encodes hexokinase-3. Similar to hexokinases-1 and hexokinases-2, this allosteric enzyme is inhibited by its product glucose 6-phosphate.

Human Hexokinase 3 References
  • Palma F. et al., 1996, Mol Cell Biochem. 155: 23-9.
  • Furuta H. et al.,1996, Genomics. 36 (1): 206-9.
  • Colosimo A. et al.,1996, Cytogenet Cell Genet. 74 (3): 187-8.
  • Product nameProduct name
    Size / Price
    Catalog: 11016-H20B-50
    List Price:   (Save )
    Price:      [How to order]
    AvailabilityIn StockShipping instructions