|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Mouse PRLR ORF mammalian expression plasmid, C-GFPSpark tag||MG50457-ACG|
|Mouse PRLR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50457-ACR|
|Mouse PRLR ORF mammalian expression plasmid, C-Flag tag||MG50457-CF|
|Mouse PRLR ORF mammalian expression plasmid, C-His tag||MG50457-CH|
|Mouse PRLR ORF mammalian expression plasmid, C-Myc tag||MG50457-CM|
|Mouse PRLR ORF mammalian expression plasmid, C-HA tag||MG50457-CY|
|Mouse PRLR Gene cDNA clone plasmid||MG50457-M|
|Mouse PRLR ORF mammalian expression plasmid, N-Flag tag||MG50457-NF|
|Mouse PRLR ORF mammalian expression plasmid, N-His tag||MG50457-NH|
|Mouse PRLR ORF mammalian expression plasmid, N-Myc tag||MG50457-NM|
|Mouse PRLR ORF mammalian expression plasmid, N-HA tag||MG50457-NY|
|Mouse PRLR natural ORF mammalian expression plasmid||MG50457-UT|
|Learn more about expression Vectors|
Prolactin receptor (PRLR) is a single-pass transmembrane receptor belonging to the type â… cytokine receptor superfamily, and contains two fibronectin type-â…¢ domains. All class 1 ligands activate their respective receptors by clustering mechanisms. Ligand binding results in the transmembrane PRLR dimerization, followed by phosphorylation and activation of the molecules invloved in the signaling pathways, such as Jak-STAT, Ras/Raf/MAPK. The PRLR contains no intrinsic tyrosine kinase cytoplasmic domain but associates with a cytoplasmic tyrosine kinase, JAK2. PRLR mainly serves as the receptor for the pituitary hormone prolactin (PRL), a secreted hormone that affects reproduction and homeostasis in vertebrates. PRLR can be regulated by an interplay of two different mechanisms, PRL or ovarian steroid hormones independently or in combination in a tissue-specific manner. The role of the hormone prolactin (PRL) in the pathogenesis of breast cancer is mediated by its cognate receptor (PRLR). Ubiquitin-dependent degradation of the PRLR that negatively regulates PRL signaling is triggered by PRL-mediated phosphorylation of PRLR on Ser349 followed by the recruitment of the beta-transducin repeats-containing protein (beta-TrCP) ubiquitin-protein isopeptide ligase. which altered PRLR stability may directly influence the pathogenesis of breast cancer.