After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

Human CD8A / MAL Protein (His Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Human CD8A Protein Product Information
Synonym:CD8A, CD8, Leu2, MAL, p32
Protein Construction:A DNA sequence encoding the extracellular domain of human CD8A (NP_001759.3) (Met 1-Asp 182) was expressed with a C-terminal polyhistidine tag.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human CD8A Protein QC Testing
Purity:> 92 % as determined by SDS-PAGE
Bio-Activity:1. Measured by its ability to bind biotinylated recombinant human B2M in a functional ELISA.
2. Measured by its ability to bind biotinylated recombinant human FCGRT+B2M in a functional ELISA.
3. Measured by its ability to bind biotinylated recombinant human LCK in a functional ELISA.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Ser 22
Molecule Mass:The recombinant human CD8A consists of 172 amino acids and predicts a molecular mass of 19 kDa. As a result of glycosylation, rh CD8A migrates as an approximately 28 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human CD8A Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human CD8A Protein SDS-PAGE
Human CD8A / MAL Protein (His Tag) SDS-PAGE
Other CD8A Recombinant Protein Products
CD8a / Lyt2 Background

Human T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I  membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

Human CD8a / Lyt2 References
References Devine, L. et al., 2000, J Immunol. 164 (2): 833-8. Arcaro, A. et al., 2000, J Immunol. 165 (4): 2068-76. Saha, K. et al., 2001, Nat Med. 7 (1): 65-72. Romero, P. et al., 2005, Eur J Immunol. 35 (11): 3092-4.
Product nameProduct name
Size / Price
Catalog: 10980-H08H-10
List Price:   (Save )
Price:      [How to order]
AvailabilityIn StockShipping instructions