|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat MFGE8 ORF mammalian expression plasmid, C-GFPSpark tag||RG80522-ACG|
|Rat MFGE8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80522-ACR|
|Rat MFGE8 ORF mammalian expression plasmid, C-Flag tag||RG80522-CF|
|Rat MFGE8 ORF mammalian expression plasmid, C-His tag||RG80522-CH|
|Rat MFGE8 ORF mammalian expression plasmid, C-Myc tag||RG80522-CM|
|Rat MFGE8 ORF mammalian expression plasmid, C-HA tag||RG80522-CY|
|Rat MFGE8 ORF mammalian expression plasmid, N-Flag tag||RG80522-NF|
|Rat MFGE8 ORF mammalian expression plasmid, N-His tag||RG80522-NH|
|Rat MFGE8 ORF mammalian expression plasmid, N-Myc tag||RG80522-NM|
|Rat MFGE8 ORF mammalian expression plasmid, N-HA tag||RG80522-NY|
|Rat MFGE8 Gene cDNA clone plasmid||RG80522-U|
|Rat MFGE8 natural ORF mammalian expression plasmid||RG80522-UT|
|Learn more about expression Vectors|
MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5/8 type C domains. It also contains a phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 has an opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner.