|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat GOT1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80493-ACG|
|Rat GOT1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80493-ACR|
|Rat GOT1 ORF mammalian expression plasmid, N-GFPSpark tag||RG80493-ANG|
|Rat GOT1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80493-ANR|
|Rat GOT1 ORF mammalian expression plasmid, C-Flag tag||RG80493-CF|
|Rat GOT1 ORF mammalian expression plasmid, C-His tag||RG80493-CH|
|Rat GOT1 ORF mammalian expression plasmid, C-Myc tag||RG80493-CM|
|Rat GOT1 ORF mammalian expression plasmid, C-HA tag||RG80493-CY|
|Rat GOT1 ORF mammalian expression plasmid, N-Flag tag||RG80493-NF|
|Rat GOT1 ORF mammalian expression plasmid, N-His tag||RG80493-NH|
|Rat GOT1 ORF mammalian expression plasmid, N-Myc tag||RG80493-NM|
|Rat GOT1 ORF mammalian expression plasmid, N-HA tag||RG80493-NY|
|Rat GOT1 Gene cDNA clone plasmid||RG80493-U|
|Rat GOT1 natural ORF mammalian expression plasmid||RG80493-UT|
|Learn more about expression Vectors|
Aspartate aminotransferase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, aspartate aminotransferase and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. There is a rare in-frame deletion in aspartate aminotransferase gene, which inactivates cytosolic aspartate aminotransferase(cAST) enzyme in the Old Order Amish. This may help to understand structure and function of the enzyme and would be useful for predicting serum aspartate AST levels.