|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat S100A11 ORF mammalian expression plasmid, C-GFPSpark tag||RG80466-ACG|
|Rat S100A11 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80466-ACR|
|Rat S100A11 ORF mammalian expression plasmid, N-GFPSpark tag||RG80466-ANG|
|Rat S100A11 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80466-ANR|
|Rat S100A11 ORF mammalian expression plasmid, C-Flag tag||RG80466-CF|
|Rat S100A11 ORF mammalian expression plasmid, C-His tag||RG80466-CH|
|Rat S100A11 ORF mammalian expression plasmid, C-Myc tag||RG80466-CM|
|Rat S100A11 ORF mammalian expression plasmid, C-HA tag||RG80466-CY|
|Rat S100A11 ORF mammalian expression plasmid, N-Flag tag||RG80466-NF|
|Rat S100A11 ORF mammalian expression plasmid, N-His tag||RG80466-NH|
|Rat S100A11 ORF mammalian expression plasmid, N-Myc tag||RG80466-NM|
|Rat S100A11 ORF mammalian expression plasmid, N-HA tag||RG80466-NY|
|Rat S100A11 Gene cDNA clone plasmid||RG80466-U|
|Rat S100A11 natural ORF mammalian expression plasmid||RG80466-UT|
|Learn more about expression Vectors|
Protein S100-A11, also known as S100 calcium-binding protein A11, S100A11 and MLN70, is a member of the S-100 family. S100A11 is widely expressed in multiple tissues, and is located in cytoplasm, nucleus, and even cell periphery. S100A11 exists as a non-covalent homodimer with an antiparallel conformation. Ca(2+) binding to S100A11 would trigger conformational changes which would expose the hydrophobic cleft of S100A11 and facilitate its interaction with target proteins. As a dual cell growth mediator, S100A11 acts as either a tumor suppressor or promoter in many different types of tumors and would play respective roles in influencing the proliferation of the cancer cells. In the nucleus, S100A11 suppresses the growth of keratinocytes through p21 (CIP1/WAF1) activation and induces cell differentiation. S100A11 is also a novel diagnostic marker in breast carcinoma.