|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IL13 ORF mammalian expression plasmid, C-GFPSpark tag||RG80460-ACG|
|Rat IL13 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80460-ACR|
|Rat IL13 ORF mammalian expression plasmid, C-Flag tag||RG80460-CF|
|Rat IL13 ORF mammalian expression plasmid, C-His tag||RG80460-CH|
|Rat IL13 ORF mammalian expression plasmid, C-Myc tag||RG80460-CM|
|Rat IL13 ORF mammalian expression plasmid, C-HA tag||RG80460-CY|
|Rat IL13 Gene cDNA clone plasmid||RG80460-G|
|Rat IL13 ORF mammalian expression plasmid, N-Flag tag||RG80460-NF|
|Rat IL13 ORF mammalian expression plasmid, N-His tag||RG80460-NH|
|Rat IL13 ORF mammalian expression plasmid, N-Myc tag||RG80460-NM|
|Rat IL13 ORF mammalian expression plasmid, N-HA tag||RG80460-NY|
|Rat IL13 natural ORF mammalian expression plasmid||RG80460-UT|
|Learn more about expression Vectors|
Interleukin 13 (IL-13) is a single-chain glycosylated polypeptide, which belongs to the IL-13/IL-4 family. IL-13 protein is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 exerts its effects through a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains (IL-13 Rα1 and IL-13 Rα2). As a cytokine, IL-13 protein is critical in regulating inflammatory, immune responses and diseases. In addition, it inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. IL-13 protein and antibody is more importantly implicated as a central mediator of immunoregulatory processes in various cell types.