|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IL22RA1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80453-ACG|
|Rat IL22RA1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80453-ACR|
|Rat IL22RA1 ORF mammalian expression plasmid, C-Flag tag||RG80453-CF|
|Rat IL22RA1 ORF mammalian expression plasmid, C-His tag||RG80453-CH|
|Rat IL22RA1 ORF mammalian expression plasmid, C-Myc tag||RG80453-CM|
|Rat IL22RA1 ORF mammalian expression plasmid, C-HA tag||RG80453-CY|
|Rat IL22RA1 Gene cDNA clone plasmid||RG80453-G|
|Rat IL22RA1 ORF mammalian expression plasmid, N-Flag tag||RG80453-NF|
|Rat IL22RA1 ORF mammalian expression plasmid, N-His tag||RG80453-NH|
|Rat IL22RA1 ORF mammalian expression plasmid, N-Myc tag||RG80453-NM|
|Rat IL22RA1 ORF mammalian expression plasmid, N-HA tag||RG80453-NY|
|Rat IL22RA1 natural ORF mammalian expression plasmid||RG80453-UT|
|Learn more about expression Vectors|
IL-22R belongs to the type II cytokine receptor family. It contains 2 fibronectin type-III domains and is expressed in colon, liver, lung, pancreas and kidney. IL-22R also can be expressed in keratinocytes of normal skin as well as in psoriatic skin lesion. Overexpression of IL-22R can be detected in synovial fluid cells from rheumatoid arthritis and spondyloarthropathy patients. IL-22R is a component of the receptor for IL20, IL22 and IL24. The component of IL-22R formed by IL22RA1 and IL10RB enables IL22 signaling via JAK/STAT pathways. IL22 also induces activation of MAPK1/MAPK3 and Akt kinases pathways. Component of one of the receptor for IL20 and IL24 formed by IL22RA1 and IL20RB also signaling through STATs activation. IL-22R mediates IL24 antiangiogenic activity as well as IL24 inhibitory effect on endothelial cell tube formation and differentiation.