|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IL20 ORF mammalian expression plasmid, C-GFPSpark tag||RG80422-ACG|
|Rat IL20 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80422-ACR|
|Rat IL20 ORF mammalian expression plasmid, C-Flag tag||RG80422-CF|
|Rat IL20 ORF mammalian expression plasmid, C-His tag||RG80422-CH|
|Rat IL20 ORF mammalian expression plasmid, C-Myc tag||RG80422-CM|
|Rat IL20 ORF mammalian expression plasmid, C-HA tag||RG80422-CY|
|Rat IL20 Gene cDNA clone plasmid||RG80422-G|
|Rat IL20 ORF mammalian expression plasmid, N-Flag tag||RG80422-NF|
|Rat IL20 ORF mammalian expression plasmid, N-His tag||RG80422-NH|
|Rat IL20 ORF mammalian expression plasmid, N-Myc tag||RG80422-NM|
|Rat IL20 ORF mammalian expression plasmid, N-HA tag||RG80422-NY|
|Rat IL20 natural ORF mammalian expression plasmid||RG80422-UT|
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IL20/Interleukin-20 belongs to the IL-10 family. It is a cytokine structurally related to interleukin 10. IL20/Interleukin-20 can be detected in skin, trachea, and other tissues. It is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells.It has been shown that interleukin-20 transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. It may be involved in epidermal function and psoriasis. It also regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL20/Interleukin-20 also causes cell expansion of multipotential hematopoietic progenitor cells. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.