|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat NTRK1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80404-ACG|
|Rat NTRK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80404-ACR|
|Rat NTRK1 ORF mammalian expression plasmid, C-Flag tag||RG80404-CF|
|Rat NTRK1 ORF mammalian expression plasmid, C-His tag||RG80404-CH|
|Rat NTRK1 ORF mammalian expression plasmid, C-Myc tag||RG80404-CM|
|Rat NTRK1 ORF mammalian expression plasmid, C-HA tag||RG80404-CY|
|Rat NTRK1 Gene cDNA clone plasmid||RG80404-G|
|Rat NTRK1 ORF mammalian expression plasmid, N-Flag tag||RG80404-NF|
|Rat NTRK1 ORF mammalian expression plasmid, N-His tag||RG80404-NH|
|Rat NTRK1 ORF mammalian expression plasmid, N-Myc tag||RG80404-NM|
|Rat NTRK1 ORF mammalian expression plasmid, N-HA tag||RG80404-NY|
|Rat NTRK1 natural ORF mammalian expression plasmid||RG80404-UT|
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TRKA is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors. The presence of NTRK1 leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. It was originally identified as an oncogene as it is commonly mutated in cancers, particularly colon and thyroid carcinomas. TRKA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. NTRK1 has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma.