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Human TIGIT / VSTM3 Protein (His Tag)

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Human TIGIT/VSTM3 Protein Product Information
Synonym:DKFZp667A205, FLJ39873, VSIG9, VSTM3, WUCAM, TIGIT
Protein Construction:A DNA sequence encoding the extracellular domain of human TIGIT (NP_776160.2) (Met 1-Phe 138) with a C-terminal polyhistidine tag was expressed.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human TIGIT/VSTM3 Protein QC Testing
Purity:> 85 % as determined by SDS-PAGE
Bio-Activity:1. Measured by its ability to bind with human CD155-Fc (Cat:10109-H02H) in a functional ELISA.
2. Measured by its ability to bind with mouse PVR-Fch (Cat:50259-M03H) in a functional ELISA.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met 22
Molecule Mass:The secreted recombinant human TIGIT comprises 128 amino acids with a predicted molecular mass of 14.2 kDa.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human TIGIT/VSTM3 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human TIGIT / VSTM3 Protein (His Tag) SDS-PAGE
Other TIGIT/VSTM3 Recombinant Protein Products
TIGIT/VSTM3 Background

TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-10 and diminished the production of IL-12p40. In addition, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.

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Catalog: 10917-H08H-50
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