|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat OPCML ORF mammalian expression plasmid, C-GFPSpark tag||RG80397-ACG|
|Rat OPCML ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80397-ACR|
|Rat OPCML ORF mammalian expression plasmid, C-Flag tag||RG80397-CF|
|Rat OPCML ORF mammalian expression plasmid, C-His tag||RG80397-CH|
|Rat OPCML ORF mammalian expression plasmid, C-Myc tag||RG80397-CM|
|Rat OPCML ORF mammalian expression plasmid, C-HA tag||RG80397-CY|
|Rat OPCML Gene cDNA clone plasmid||RG80397-G|
|Rat OPCML ORF mammalian expression plasmid, N-Flag tag||RG80397-NF|
|Rat OPCML ORF mammalian expression plasmid, N-His tag||RG80397-NH|
|Rat OPCML ORF mammalian expression plasmid, N-Myc tag||RG80397-NM|
|Rat OPCML ORF mammalian expression plasmid, N-HA tag||RG80397-NY|
|Rat OPCML natural ORF mammalian expression plasmid||RG80397-UT|
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Opioid-binding Cell Adhesion Molecule (OBCAM), also known as OPCML, is a GPI-anchored cell adhesion molecule in the plasma membrane. This neuron-specific protein, consists of three immunoglobulin (Ig)-like domains anchored to the membrane through a glycosylphosphatidylinositol (GPI)-tail. OPCML also belongs to the member of the IgLON family, a subgroup of the immunoglobulin superfamily, consisting of three members, LAMP, OBCAM, and Neurotrimin. These molecules interact homophilically and heterophilically within the family, and OBCAM acts only as heterodimers with LAMP or Neurotrimin and possibly inhibits neurite outgrowth from cerebellar granule cells. OBCAM has been presumed to play a role as a cell adhesion/recognition molecule. Furthermore, the OPCML protein defects may play an important role in the carcinogenesis of cervical or ovarian cancers, and this gene is regarded as a candidate TSG (tumor suppressor gene).