|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IL23R ORF mammalian expression plasmid, C-GFPSpark tag||RG80395-ACG|
|Rat IL23R ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80395-ACR|
|Rat IL23R ORF mammalian expression plasmid, C-Flag tag||RG80395-CF|
|Rat IL23R ORF mammalian expression plasmid, C-His tag||RG80395-CH|
|Rat IL23R ORF mammalian expression plasmid, C-Myc tag||RG80395-CM|
|Rat IL23R ORF mammalian expression plasmid, C-HA tag||RG80395-CY|
|Rat IL23R Gene cDNA clone plasmid||RG80395-G|
|Rat IL23R ORF mammalian expression plasmid, N-Flag tag||RG80395-NF|
|Rat IL23R ORF mammalian expression plasmid, N-His tag||RG80395-NH|
|Rat IL23R ORF mammalian expression plasmid, N-Myc tag||RG80395-NM|
|Rat IL23R ORF mammalian expression plasmid, N-HA tag||RG80395-NY|
|Rat IL23R natural ORF mammalian expression plasmid||RG80395-UT|
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IL23R, also known as IL23 receptor, belongs to the type I cytokine receptor family, Type 2 subfamily. It contains 2 fibronectin type-III domains and is expressed by monocytes, Th1, Th0, NK and dendritic cells. Isoform 1 is specifically expressed in NK cells. IL23R associates with IL12RB1 to form the interleukin-23 receptor. It binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis. Genetic variations in IL23R are associated with inflammatory bowel disease type 17 (IBD17). IBD17 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. Genetic variations in IL23R also can cause susceptibility to psoriasis type 7.