|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat COLEC12 ORF mammalian expression plasmid, C-GFPSpark tag||RG80394-ACG|
|Rat COLEC12 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80394-ACR|
|Rat COLEC12 ORF mammalian expression plasmid, C-Flag tag||RG80394-CF|
|Rat COLEC12 ORF mammalian expression plasmid, C-His tag||RG80394-CH|
|Rat COLEC12 ORF mammalian expression plasmid, C-Myc tag||RG80394-CM|
|Rat COLEC12 ORF mammalian expression plasmid, C-HA tag||RG80394-CY|
|Rat COLEC12 Gene cDNA clone plasmid||RG80394-G|
|Rat COLEC12 ORF mammalian expression plasmid, N-Flag tag||RG80394-NF|
|Rat COLEC12 ORF mammalian expression plasmid, N-His tag||RG80394-NH|
|Rat COLEC12 ORF mammalian expression plasmid, N-Myc tag||RG80394-NM|
|Rat COLEC12 ORF mammalian expression plasmid, N-HA tag||RG80394-NY|
|Rat COLEC12 natural ORF mammalian expression plasmid||RG80394-UT|
|Learn more about expression Vectors|
CLP1, also known as COLEC12, is a scavenger receptor that displays several functions associated with host defense. It contains 1 C-type lectin domain and 3 collagen-like domains. CLP1 is strongly expressed in placenta and moderately expressed in heart, skeletal muscle, small intestine and lung. It promotes binding and phagocytosis of Gram-positive, Gram-negative bacteria and yeast. CLP1 mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. It binds to several carbohydrates including Gal-type ligands, D-galactose, L- and D-fucose, GalNAc, T and Tn antigens in a calcium-dependent manner and internalizes specifically GalNAc in nurse-like cells. It binds also to sialyl Lewis X or a trisaccharide and asialo-orosomucoid (ASOR). CLP1 may also play a role in the clearance of amyloid beta in Alzheimer disease.