|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CLEC4A3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80392-ACG|
|Rat CLEC4A3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80392-ACR|
|Rat CLEC4A3 ORF mammalian expression plasmid, N-GFPSpark tag||RG80392-ANG|
|Rat CLEC4A3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80392-ANR|
|Rat CLEC4A3 ORF mammalian expression plasmid, C-Flag tag||RG80392-CF|
|Rat CLEC4A3 ORF mammalian expression plasmid, C-His tag||RG80392-CH|
|Rat CLEC4A3 ORF mammalian expression plasmid, C-Myc tag||RG80392-CM|
|Rat CLEC4A3 ORF mammalian expression plasmid, C-HA tag||RG80392-CY|
|Rat CLEC4A3 Gene cDNA clone plasmid||RG80392-G|
|Rat CLEC4A3 ORF mammalian expression plasmid, N-Flag tag||RG80392-NF|
|Rat CLEC4A3 ORF mammalian expression plasmid, N-His tag||RG80392-NH|
|Rat CLEC4A3 ORF mammalian expression plasmid, N-Myc tag||RG80392-NM|
|Rat CLEC4A3 ORF mammalian expression plasmid, N-HA tag||RG80392-NY|
|Rat CLEC4A3 natural ORF mammalian expression plasmid||RG80392-UT|
|Learn more about expression Vectors|
CLEC4A3 contains 1 C-type lectin domain and belongs to the C-type lectin-like domain-containing (CLEC) family. Lectins are proteins that are able to recognize and bind with specific carbohydrate molecules. C-type lectins are an important group of proteins found in the immune system of animals. These lectins are named C-type because of their calcium dependent carbohydrate recognition domain (CRD). In the immune system, C-type lectins act as recognition molecules by binding to foreign microorganisms. They also promote the movement and selective adhesion of white blood cells.
The C-type lectin has a three-dimensional fold, the CRD, in which calcium ions contribute to the lectin's ability to recognize and bind carbohydrates. In the immune system, carbohydrate recognition contributes to the ability of immune cells to move from one area of the body to another. It also allows immune cells to identify and discriminate between proteins that belong to the host and those that belong to foreign organisms. There are a number of different C-type lectin subfamilies, including collectins, selectins, proteoglycans, and lymphocyte lectins.