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Human CD73 / NT5E Protein (His Tag)

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Human NT5E Protein Product Information
Synonym:FSTL1, FLJ50214, FLJ52277, FRP, FSL1
Protein Construction:A DNA sequence encoding the human NT5E (NP_002517.1) (Met 1-Lys 547) without the propeptide was expressed, fused with a polyhistidine tag at the C-terminus.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human NT5E Protein QC Testing
Purity:> 85 % as determined by SDS-PAGE
Bio-Activity:Measured by its ability to hydrolyze the 5’phosphate group from the substrate adenosine 5’monophosphate (AMP).
The specific activity is > 20,000 pmoles/min/μg.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Trp 27
Molecule Mass:The secreted recombinant human NT5E consists of 532 amino acids with the predicted molecular mass of 59.2 kDa.
Formulation:Lyophilized from sterile 20mM Tris, 120 mM NaCl, 4 mM CaCl2, 20 % glycerol, pH 7.5.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human NT5E Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human NT5E Protein SDS-PAGE
Human CD73 / NT5E Protein (His Tag) SDS-PAGE
Other NT5E Recombinant Protein Products
CD73 / NT5E Background

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.

Human CD73 / NT5E References
  • Resta R. et al., 1997, Cell Signal. 9 (2): 131-9.
  • Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90.
  • Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55.
  • Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.
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    Catalog: 10904-H08H-100
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