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Human CCNE1 / Cyclin-E1 Protein

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Human CCNE1 Protein Product Information
Protein Construction:A DNA sequence encoding the human CCNE1 (NP_001229.1) (Met 1-Ala 410) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human CCNE1 Protein QC Testing
Purity:> 90 % as determined by SDS-PAGE
Bio-Activity:Measured by its binding ability in a functional ELISA.
Immobilized human CCNE1 (Cat:10902-HNCB) at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CDK4 (Cat:10732-H09B), The EC50 of biotinylated human GST-CDK4 (Cat:10732-H09B) is 0.55-1.29 μg/ml.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Gly
Molecule Mass:The secreted recombinant human CCNE1 consists of 412 amino acids and predicts a molecular mass of 47.2 KDa. The apparent molecular mass of the protein is approximately 48 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation:Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human CCNE1 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other CCNE1 Recombinant Protein Products
Cyclin E1/CCNE1 Background

Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.

Human Cyclin E1/CCNE1 References
  • Honda R, et al. (2005) The structure of cyclin E1 / CDK2: implications for CDK2 activation and CDK2-independent roles. The EMBO Journal. 24: 452-63.
  • Geng Y, et al. (2007) Kinase-Independent Function of Cyclin E. 25(1): 127-39.
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    Catalog: 10902-HNCB-100
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