|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat MPL ORF mammalian expression plasmid, C-GFPSpark tag||RG80346-ACG|
|Rat MPL ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80346-ACR|
|Rat MPL ORF mammalian expression plasmid, C-Flag tag||RG80346-CF|
|Rat MPL ORF mammalian expression plasmid, C-His tag||RG80346-CH|
|Rat MPL ORF mammalian expression plasmid, C-Myc tag||RG80346-CM|
|Rat MPL ORF mammalian expression plasmid, C-HA tag||RG80346-CY|
|Rat MPL Gene cDNA clone plasmid||RG80346-G|
|Rat MPL ORF mammalian expression plasmid, N-Flag tag||RG80346-NF|
|Rat MPL ORF mammalian expression plasmid, N-His tag||RG80346-NH|
|Rat MPL ORF mammalian expression plasmid, N-Myc tag||RG80346-NM|
|Rat MPL ORF mammalian expression plasmid, N-HA tag||RG80346-NY|
|Rat MPL natural ORF mammalian expression plasmid||RG80346-UT|
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CD110, also known as c-MPL, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. It is expressed at a low level in a large number of cells of hematopoietic origin. C-MPL is homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. Thrombopoietin is the ligand for c-mpl. It was shown to be the major regulator of megakaryocytopoiesis and platelet formation. Defects in c-MPL are a cause of congenital amegakaryocytic thrombocytopeniawhich is a disease characterized by isolated thrombocytopenia and megakaryocytopenia with no physical anomalies. Defects in c-MPL also cause thrombocythemia type 2 and myelofibrosis with myeloid metaplasia.